The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. The ladder is ready for loading Mix the ladder well before use Take 3-5 µl of the ladder for a small size gel slot for electrophoresis Use a bit less of the ladder (2-3 µl) for Western blots Due to high amount of the protein in each ladder band, the ladder might show positive results in immunodetection of blots. Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. Includes 11 protein bands from 8 to 260 kDa (see figure). Tested in polyacrylamide gel electrophoresis and Western Blotting. The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. Easy to identify: Includes green 25 kDa and red 75kDa reference bands. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. Ready-to-use: Supplied in a loading buffer for direct loading on gels. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. Use 5-15µl, depending on the size of the wellsĪpplication: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.2 reference bands - red at ~75kDa and green at ~25kDa.Stoolfix Gut Microbiome Stabilization Kitsīullseye Pre-stained Protein Ladder, 10-180 kDa.Description The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. Beakers, Graduated Cylinders, and Flasks Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis Categories: Protein Marker/Ladder.Aluminum Foil, Parafilm, and Multiwipes.
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